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. 2022 Oct 20;8:136. doi: 10.1038/s41531-022-00388-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a The diagram depicts the workflow of our study to assess the background and specificity of commercial and homemade pS129 antibodies using recombinant aSyn monomers and fibrils (WT or carrying specific PTMs such as nitration, phosphorylation and/or truncation), mammalian cell lines overexpressing aSyn, aSyn KO primary neurons, WT neurons treated with aSyn PFFs or WT mice injected with aSyn PFFs. The background and specificity of the selected pS129 antibodies were evaluated by Western blot analyses or ICC or HTS combined with confocal imaging. Panel A was created using BioRender.com. b The diagram depicts aSyn human sequence, the epitopes of each of the pS129 antibodies (whenever available), and the post-translational modifications that can occur concomitantly in the C-terminal region of aSyn. c The diagram depicts truncations that occurs in the different regions of aSyn in human brains and the appendix.