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. 2022 Oct 20;8:136. doi: 10.1038/s41531-022-00388-7

Fig. 7. Assessment of pS129 antibodies detection in brain sections of aSyn KO mice.

Fig. 7

a Brain coronal sections (50 μM thick) from different brain areas of aSyn KO mice (Amygdala, Striatum, Motor Cortex, Hippocampus and Substantia Nigra) have been examined to assess the different staining patterns of the six pS129 antibodies (pSyn#64, MJF-R13, 81A, EP1536Y, GTX, and LASH-EGT). Scale bar = 100 μM. b pS129 level of detection in the soluble and insoluble fractions extracted from the aSyn KO mice brains using the six pS129 antibodies (pSyn#64, MJF-R13, 81a, EP1536Y, GTX, and LASH-EGT). Sequential biochemical extraction was performed on different brain regions (cortical/CTX; Midbrain/MID and Striatum/STR) derived from aSyn KO mice, and the level of pS129 was assessed in the soluble (0.1% Triton-soluble fraction) and the insoluble fraction (2% SDS-soluble fraction). 40 ng of recombinant aSyn WT or phosphorylated at residue S129 (pS129, indicated by the red arrow) were used as positive controls. Membranes were counterstained for total aSyn (SYN-1 or D37A6), and actin was used as a loading control. The red arrows indicate the pS129-aSyn-positive bands. The blue arrows indicate the undefined bands. All blots were derived from the same experiment and were processed in parallel.