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. 2022 Oct 20;13:6208. doi: 10.1038/s41467-022-34017-x

Fig. 5. Interaction between the core and gate domains.

Fig. 5

a–c The core-gate domain interface in the Cl−-bound (a), SO42−-bound (b), and salicylate-bound (c) PresTS structures, viewed from the extracellular side. Putative hydrogen bonds are indicated by yellow dotted lines. d Cell membrane electric capacitance measurement in HEK293T cells expressing Q97D-, Q97E-, N447D-, N447A-, E293K-, E293A-, V353D-, V353E, V353N-, V353A-, V353I-, V353L, V353Y, V353W, and V353F-HgPres. Four to five examples in different colors are shown for each panel. Solid lines (for Q97E-, E293A-, N447A-, V353A-, V353L-, V353I-, V353F-, and V353Y-HgPres) indicate two-state Boltzmann fittings. The α, Vpk, and charge density values (mean ± S.D.) were as follows: [0.021 ± 0.006 mV−1, −59 ± 30 mV, 8 ± 3 fC/pF] for Q97E (n = 4); [0.023 ± 0.007 mV−1, −63 ± 33 mV, 7 ± 7 fC/pF] for E293A (n = 10); [0.027 ± 0.004 mV−1, −65 ± 8 mV, 4 ± 2 fC/pF] for N447A (n = 5); [0.036 ± 0.004 mV−1, 51 ± 8 mV, 8 ± 4 fC/pF] for V353A (n = 6); [0.034 ± 0.011 mV−1, −92 ± 18 mV, 10 ± 6 fC/pF] for V353L (n = 6); [0.031 ± 0.004 mV−1, −63 ± 20 mV, 8 ± 4 fC/pF] for V353I (n = 6); and [0.022 ± 0.003 mV−1, 89 ± 6 mV, 13 ± 5 fC/pF] for V353F (n = 6). Source data are provided as a Source Data file.