Table 1.
Effect of the solvent in SLE procedure applied for extraction antimicrobials from soil.
| Antimicrobials recovery (%) (SD (%)) | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Solvent | TC | OTC | DOX | CIP | LVF | ENF | TYL | TRI | VAN | MET | CLR | CLD | SMX | SFD |
| Citrate buffer (pH = 4):ACN (1:1; v/v) | 31.7 (2.1) | 26.3 (1.7) | 30.6 (5.4) | 10.8 (2.0) | 15.8 (2.1) | 12.8 (3.7) | 103.2 (7.8) | 98.0 (8.5) | 58.1 (4.9) | 100.5 (8.8) | 89.6 (7.9) | 110.1 (12.2) | 88.1 (9.0) | 104.0 (3.9) |
| Citrate buffer (pH = 4):MeOH (1:1; v/v) | 27.7 (2.2) | 31.3 (2.1) | 33.1 (1.5) | 5.2 (1.5) | 4.7 (0.5) | 4.7 (0.7) | 86.2 (6.7) | 78.1 (4.4) | 65.1 (5.9) | 83.0 (17.6) | 79.6 (4.6) | 91.7 (12.9) | 78.4 (1.9) | 97.8 (3.5) |
| 0.1% FA in MeOH | – | – | – | 1.4 (0.1) | 11.7 (1.3) | 2.1 (0.5) | 88.5 (10.2) | 89.0 (2.3) | – | 85.4 (8.7) | 86.7 (1.6) | 90.0 (14.0) | 68.0 (4.5) | 76.3 (6.3) |
| MeOH:AcEt (1:1; v/v) | – | – | – | 0.0 (0.0) | 0.0 (0.0) | 0.0 (0.0) | 97.5 (10.9) | 84.9 (5.3) | – | 103.9 (11.5) | 67.4 (2.8) | 98.4 (11.1) | 72.0 (6.1) | 69.3 (5.1) |
| 0.2 M NaOH in H2O | – | – | – | 39.1 (2.1) | 29.9 (1.2) | 53.4 (9.6) | 33.9 (0.5) | 94.0 (4.8) | 117.4 (8.6) | 80.5 (8.9) | 61.3 (4.2) | 79.8 (6.4) | 69.1 (4.2) | 63.8 (2.2) |
| 2% NH3 in MeOH | – | – | – | 5.1 (0.6) | 5.1 (0.7) | 4.0 (0.7) | 61.1 (3.6) | 93.7 (6.2) | 3.5 (0.1) | 53.6 (6.3) | 48.9 (3.2) | 68.2 (8.8) | 46.7 (5.8) | 50.8 (4.3) |
| ACN:0.1% FA in H2O (1:1; v/v) | – | – | – | 3.6 (0.6) | 6.2 (0.7) | 5.5 (0.3) | 104.6 (4.6) | 91.1 (12.1) | 66.2 (6.4) | 77.5 (8.6) | 83.0 (3.8) | 72.3 (10.5) | 84.0 (2.0) | 63.3 (0.6) |
| Citrate buffer (pH = 2):ACN (1:1; v/v) | 15.5 (1.6) | 14.1 (1.8) | 1.8 (0.28) | 9.4 (1.2) | 13.1 (1.6) | 14.7 (2.6) | 68.1 (4.1) | 90.1 (4.6) | 86.6 (1.6) | 83.1 (13.3) | 54.1 (4.3) | 87.5 (14.1) | 54.6 (2.3) | 96.0 (3.7) |
| McIlvaine buffer (pH = 4):MeOH (1:1; v/v) | 48.7 (4.7) | 48.1 (5.7) | 44.7 (5.5) | 3.9 (0.2) | 5.5 (0.7) | 2.6 (0.2) | 71.2 (5.8) | 63.5 (7.5) | 75.2 (8.3) | 85.6 (6.4) | 90.3 (7.2) | 90.2 (6.8) | 96.1 (7.1) | 84.6 (12.2) |
| McIlvaine buffer (pH = 4):ACN (1:1; v/v) | 86.7 (9.5) | 79.7 (7.3) | 83.7 (7.7) | 22.9 (2.4) | 25.4 (4.7) | 29.3 (2.7) | 79.1 (2.5) | 85.3 (4.9) | 71.0 (10.5) | 74.9 (3.9) | 89.6 (7.9) | 82.9 (11.0) | 84.1 (8.2) | 81.7 (4.9) |
| MeOH:ACN:0.1 M EDTA:McIlvaine buffer (pH = 4) (30:20:25:25; v/v/v/v) | 57.9 (7.9) | 59.2 (6.2) | 93.9 (8.7) | 14.2 (1.7) | 26.8 (3.3) | 15.9 (1.2) | 81.7 (3.1) | 81.8 (3.5) | 96.7 (9.3) | 83.3 (3.1) | 86.9 (6.6) | 85.9 (14.5) | 95.5 (55.7) | 88.2 (2.7) |
| ACN:McIlvaine buffer (pH = 4):0.1 M EDTA (2:1:1; v/v/v) | 70.6 (6.0) | 67.4 (8.3) | 88.3 (4.4) | 15.8 (0.8) | 16.5 (4.5) | 20.9 (1.2) | 79.4 (1.0) | 90.9 (6.1) | 92.9 (9.5) | 55.8 (3.6) | 80.4 (10.6) | 88.2 (10.9) | 89.0 (3.7) | 59.0 (2.98) |
| 0.2 M NaOH in H2O:MeOH (1:1; v/v) | 2.9 (0.2) | 2.9 (0.2) | – | 75.3 (5.0) | 54.8 (12.2) | 85.6 (5.1) | 40.2 (5.4) | 98.5 (5.0) | 79.5 (9.2) | 62.2 (3.4) | 81.7 (2.2) | 71.08 (6.4) | 89.8 (14.8) | 115.0 (16.9) |
| 0.2 M NaOH in H2O:ACN (1:1; v/v) | 5.4 (0.7) | 5.8 (1.1) | 6.3 (0.8) | 79.5 (3.9) | 96.8 (4.8) | 86.9 (4.0) | 70.0 (2.3) | 104.9 (14.6) | 96.3 (1.7) | 59.6 (14.0) | 80.1 (14.9) | 90.5 (5.7) | 94.4 (11.6) | 82.6 (6.6) |
| 0.2 M NaOH in H2O:acetone (1:1; v/v) | 0.7 (0.1) | 1.4 (0.1) | – | 85.4 (10.9) | 107.3 (17.0) | 86.5 (7.9) | 2.34 (0.3) | 60.8 (9.4) | 66.9 (7.3) | 64.3 (5.9) | 81.8 (9.6) | 72.9 (2.1) | 88.2 (11.7) | 88.0 (11.9) |
ACN—acetonitrile, FA—formic acid, AcEt—ethyl acetate, “–”—no data (no peak in the chromatogram).