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. 2022 Oct 14;115(11):1363–1378. doi: 10.1007/s10482-022-01779-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Maximum likelihood phylogenetic tree of L. kluyveri (LkUra1; dihydroorotate dehydrogenase) fungal orthologs. A search for orthologs of Class I-A dihydroorotate dehydrogenases using Lachancea kluyveri LkUra1 (UniProt KB accession number: Q7Z892) as query resulted in 203 proteins (Dataset S01). Sequence identifiers corresponding to the phyla Basidiomycota, Ascomycota and Mucoromycota are indicated in color. Sequences that were functionally analysed in this study, as well as the the characterised Ura1 proteins of L. kluyveri (Gojković et al. 2004), and S. cerevisiae (Zameitat et al. 2007) are indicated with the corresponding species name. The tree was midpoint rooted, and the raw phylogenetic tree is provided in Dataset S02. Bootstrap values and species can be accessed from: https://itol.embl.de/tree/8384480104951618225493