Table 1.

Complementation by uracil (ura) or dihydrouracil (dhu) of uracil-auxotrophic S. cerevisiae strains expressing fungal proteins with homology to Ura1

Strain	Relevant genotype	SMD	SMD + ura	SMD + dhu
IMX585	URA1	0.37 ± 0.00	0.37 ± 0.00	0.37 ± 0.01
IMK824	ura1Δ	n.a	0.35 ± 0.00	n.a
IMI433	ura1Δ::Aadho	n.a	0.37 ± 0.00	0.34 ± 0.00
IMI434	ura1Δ::Scdho	n.a	0.35 ± 0.00	0.34 ± 0.01

Specific growth rates were measured in duplicate shake-flask cultures for each combination of strain and medium composition, data are represented as average ± mean deviation. SMD, synthetic medium with glucose; SMD + ura, SMD supplemented with 1.5 g L⁻¹ uracil; SMD + dhu, SMD supplemented with 2.5 g L⁻¹ dihydrouracil. n.a. (not applicable) indicates that no exponential growth was observed