Table 3.
Primers used for the amplification and sequencing of the ssrDNA of the new Henneguya spp.
| Primer | Sequence 5′–3′ | Paired with | Reference |
|---|---|---|---|
| Erib1 | ACCTGGTTGATCCTGCCAG | Act1r | Barta et al.39 |
| Myxgen4F | GTGCCTTGAATAAATCAGAG | Erib10 | Kent et al.40 |
| Act1r | AATTTCACCTCTCGCTGCCA | Erib1 | Hallett and Diamant41 |
| Erib10 | CTTCCGCAGGTTCACCTACGG | Myxgen4F | Barta et al.39 |
| MB5 | GGTGATGATTAACAGGAGCGGT | MX3 | Eszterbauer42 |
| MX3 | CCAGGACATCTTAGGGCATCACAGA | MB5 | Andree et al.32 |
Cycling conditions (for all primers): Initial denaturation at 95 °C for 3 min, followed by 35 cycles of 95 °C for 1 min, annealing at 55 °C for 45 s, 72 °C for 2 min, and a final extension at 72 °C for 7 min.