FIGURE 3.
Treatment with Ginsenoside Rc Effectively Alleviates APAP Overdose-Induced Inflammation and antiapoptosis (A) The TUNEL assay was performed to measure the apoptosis effect in APAP-induced mice; (B) Relative expression of mRNA associated with antiapoptosis (Caspase, Bcl2/Bax); (C) Ginsenoside Rc treatment inhibits the expression of the Bcl2 protein and restrains the expression of Bax in mice; (D) Relative expression of mRNA associated with inflammation (Il-6, Il-1β, TNF-α); (E) The protein levels of F4/80 and CD11b were determined using immunofluorescence (1000X). The nuclei were stained with DAPI; (F–H) Serum pro-inflammatory cytokines, including TNF-α, IL-1β, and IL-6, were decreased in APAP-induced mice after Ginsenoside Rc treatment. Data are means ± SEM; n = 5-8/group. *, p < 0.05, **; p < 0.01, ***; p < 0.001.