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. 2022 Oct 7;13:1027731. doi: 10.3389/fphar.2022.1027731

FIGURE 5.

FIGURE 5

The absence of FXR abrogated the hepatoprotective effects of Ginsenoside Rc against APAP overdose-induced ALI in MPHs. (A) The TUNEL assay was performed to measure the apoptosis effect in FXR−/− MPHs;(B) Ginsenoside Rc administration did not alter the genes involved in apoptosis in FXR−/− MPHs; (C) ROS fluorescence in FXR−/− MPHs; (D) Expression of mRNA levels of oxidative stress genes (Sod2,Gclc, Gclm) in FXR−/− MPHs; (E) Immunofluorescence for NFκB- p65 (400×); (F) Ginsenoside Rc administration does not alter the genes involved in inflammation in FXR−/− MPHs (Il-6, Il-1β, Tnf-α); (G) The ELISA assay showed that Ginsenoside Rc treatment does not alter the NAPQI levels in FXR−/− MPHs. (H) Ginsenoside Rc administration does not alter the genes involved in APAP-metabolizing enzymes in FXR−/− MPHs (Cyp3a11, Cyp2E, Ugt1a11); Data are means ± SEM; n = 5-8/group. *,p < 0.05, **; p < 0.01, ***; p < 0.001.