Figure 5.

GLDC is negatively regulated by miR-30e in TNBC. (A) There was no significant difference on the promoter methylation level of GLDC between TNBC and normal breast tissues. (B) The schematic diagram of the protocol utilized for identifying the potential functional regulator of GLDC. (C) The expression of GLDC was negatively correlated with miR-30e expression in the same TNBC tissues. (D) The potential binding site of miR-30e conserved in the 3′UTR of GLDC. (E) Treatment with miR-30e led to the decreased luciferase activity of the WT (wild type) group. (F, G) MiR-30e significantly depressed the mRNA (F) and protein (G) expression of GLDC in TNBC cells. (H, I) Treatment with anti-miR-30e led to the increased expression of GLDC at both mRNA (H) and protein (I) levels. *P < 0.05.