Skip to main content
. 2022 Oct 7;10:993298. doi: 10.3389/fbioe.2022.993298

FIGURE 3.

FIGURE 3

Cre mRNA-LNPs injected IV into CRE reporter mice elicits reporter gene activation in the liver. (A) Schematic representation of the reporter mouse strain, B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J (CRE-mouse). (B) CRE mice were injected IV and euthanized 24 h later. Ex vivo imaging of tissues are shown for: Group 1, CRE mice injected with Cre mRNA-LNPs; Group 2, CRE mice injected with NaCl; Group 3, C57BL/6J mice injected with Cre mRNA-LNPs; Group 4, C57BL/6J mice injected with NaCl. (C) Quantification of tdTomato fluorescent signal obtained for livers after ex vivo imaging. (D) Western blot analysis of tdTomato protein (visualized by anti-RFP antibody; RFP, Red Fluorescent Protein) in CRE mouse livers normalized to β-actin loading control. (E) Quantitation of tdTomato protein bands normalized to β-actin loading control. Results are the means ± SEM for groups of mice (n = 5) injected with Cre mRNA-LNPs or saline. Statistically significant difference to the saline control: ****p < 0.0001 (two-tailed Student’s t-test).