Figure 4.

TCRKO CAR T cells maintain effector potency after repetitive tumor re-challenge. (A) Scheme of tumor re-challenge assay. CAR T cells were cocultured with Namalwa cells (E:T = 1:2) and rechallenged every two days. (B) Graph showing number of remaining tumor cells quantified every 48h. (C) T cell fold expansion after every encounter related to the initial numbers of T cells seeded. (D) Percentage of T_N/T_SCM +T_CM of WT ARI-0001 and TCRKO ARI-0001 after the different challenges with Namalwa cells (4 independent donors, n=4). (E) Graphs showing the percentage of expression of 0(black), 1(blue), 2(green) and 3(gray) exhaustion markers/inhibitory receptors (IR) (TIM3, PD1 and LAG3) in WT (left) and TCRKO (right) ARI-0001 CAR-T cells before encounter (basal-top) and after one (1^st encounter-middle) and two encounters (2^nd encounter-bottom) with Namalwa cells (3 independent donors, n=3). Graphs show mean ± SEM.