. 2022 Aug 15;2(5):521–528. doi: 10.1021/acsbiomedchemau.2c00032

Table 1. Steady-State Kinetic Parameters Derived for MpPCO and KnPCO Activity toward AtRAP2_2-15 and MpERF-like_2-15^a.

enzyme/substrate	k_cat, s^–1	K_M, μM	k_cat/K_M, M^–1 s^–1	V_max, μmol min^–1 mg^–1
MpPCO/AtRAP2_2–15	8.2 ± 0.5	11 ± 1.1	7.45 x10⁵	17.6 ± 1.1
MpPCO_c/ MpERF_2–15	7.9 ± 0.4	509 ± 75	1.55 x10⁴	17.0 ± 0.9
KnPCO/AtRAP2_2–15	18.0 ± 1.2	914 ± 144	1.97 x10⁴	36.6 ± 2.4
KnPCO/MpERF_2–15	43.4 ± 4.3	930 ± 223	4.67 x10⁴	87.9 ± 8.7

Experiments were conducted at atmospheric O₂.

Table 1. Steady-State Kinetic Parameters Derived for MpPCO and KnPCO Activity toward AtRAP22-15 and MpERF-like2-15a.