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Reactions of wild type and variant LipS1 proteins with the 8-mercaptooctanoyllysyl peptide substrate. WT LipS1 (blue), C54A LipS1 (red), C65A LipS1 (black), C230A LipS1 (maroon), C276A LipS1 (purple), C277A LipS1 (cyan), and H159A LipS1 (gray). Reactions contained 10 μM WT LipS1 or indicated variant, 300 μM 8-mercaptooctanoyllysyl peptide, 0.5 mM SAM, and 1 mM dithionite and were conducted at 45 °C in triplicate. Error bars represent one standard deviation from the mean.
