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. 2022 Jul 14;2(5):509–520. doi: 10.1021/acsbiomedchemau.2c00018

Figure 12.

Figure 12

Reactions of wild type LipS2 (blue), C85A LipS2 (red), C276A LipS2 (orange), C279A LipS2 (cyan), and H178A LipS2 (purple) with the octanoyllysyl peptide substrate. The reactions contained 10 μM wt LipS2 or 10 μM indicated variant, 300 μM octanoyllysyl peptide substrate, 0.5 mM SAM, and 1 mM dithionite and were conducted at 45 °C in triplicate. Error bars represent one standard deviation from the mean.