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LipS1 attaches sulfur to an 8-mercaptooctanoyllysyl peptide only. The LipS1 reaction with (A) octanoyllysyl peptide (peptide 1), (B) 6-mercaptooctanoyllysyl peptide (peptide 2), and (C) 8-mercaptooctanoyllysyl peptide (peptide 3). The reactions contained 10 μM LipS1, 300 μM respective substrate, 1 mM dithionite, and 0.5 mM SAM. Reactions were conducted at 45 °C in triplicate. Error bars represent one standard deviation from the mean.
