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LipS2 attaches sulfur to an octanoyllysyl peptide only. The LipS2 reaction with (A) 6-mercaptooctanoyllysyl peptide, (B) 8-mercaptooctanoyllysyl peptide, and (C) octanoyllysyl peptide. The reactions contained 10 μM LipS2, 300 μM respective substrate, 1 mM dithionite, and 0.5 mM SAM. Reactions were conducted at 45 °C in triplicate. Error bars represent one standard deviation from the mean.
