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Investigation of catalytic acid. (A) Scheme for dephosphorylation to the detected alcohols. (B) Extracted ion (m/z = 275) chromatograms from GC–MS analysis of extracts from E. coli engineered to produce 1 and express the wild-type (WT) or indicated mutant of MtHPS. (C) TerDockin-based depiction of activating hydrogen bonds (dotted yellow lines) formed between D293 and T296, as well as H341 and D295, which acts as the general acid.20
