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Y479 acts as the catalytic base. (A) TerDockin-based depiction of the proximity of Y479 hydroxyl to hydrogen on C6 of final intermediate D removed to produce 2.20 (B) Extracted ion (m/z = 275) chromatogram from GC–MS analysis of the extract from E. coli engineered to produce 1 and express MtHPS:Y479F. (C) Structure of alternative product 3, labda-8,13-dienyl diphosphate (PP).
