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Y328 does not serve as the secondary catalytic base. (A) Extracted ion (m/z = 275) chromatograms from GC–MS analysis of the extract from E. coli engineered to produce 1 and express indicated mutants of MtHPS, leading to alternative products. (B) TerDockin-based depiction of the distance from Y328 to the hydrogen on C9 of intermediate A (diphosphate not depicted for clarity) removed to produce 3.20
