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. 2022 Jun 28;2(5):490–498. doi: 10.1021/acsbiomedchemau.2c00023

Figure 6.

Figure 6

Substitution of histidine for F171 enables production of CPP (4). (A) Extracted ion (m/z = 275) chromatograms from GC–MS analysis of extracts from E. coli engineered to produce 1 and expressing indicated mutants of MtHPS. (B) Structures of alternative products 4, labda-8(17),13-dienyl diphosphate (PP), and 5, labda-7,13-dienyl PP (*, unknown product). (C) Depiction of the proximity of H171 (from MtHPS:F171H) to C17 of intermediate A (with diphospahte replaced by single blue atom for clarity), which is deprotonated to produce 4, indicated by TerDockin analysis. (D) Mass spectra of the unknown product (*).