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Concentration effect of 14c on intracellular SphK2 inhibition. U937 cells were incubated with 1 μM FTY720 and varying concentrations of 14c. Following incubation, U937 cells were harvested by centrifugation and lysed, and the levels of (A) S1P and (B) FTY720-P were quantified via LC-MS/MS. Each experiment was performed in duplicate.
