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. 2022 Aug 24;210(1):39–52. doi: 10.1093/cei/uxac074

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Figure 4: — Flow cytometry analysis of the expression of NK1R on mouse monocytes and B cells. Monocytes were represented as CD11b⁺Ly-6C/G⁺ cells. B cells were represented as CD19⁺ cells. Mice were randomly selected into OVA-sensitized and OVA-challenged group (OVA + OVA), OVA-sensitized and normal saline (NS) challenged group (OVA + NS), and NS control group (NS + NS). (A) and (B) showed the gating strategies of monocytes and B cells in the blood of OVA-sensitized and non-sensitized mice, respectively. In both (A) and (B), (a) represented gating strategies of monocytes and B cells, while (b) and (c) represented the allergic rhinitis (AR) and allergic asthma (AA) models, respectively. (C) and (D) demonstrated changes in the percentages of NK1R⁺ monocytes of normal saline (NS) and OVA-sensitized AR and AA mice. (E) and (F) illustrated changes in the percentages of NK1R⁺ B cells of NS and OVA-sensitized AR and AA mice. A total of 7 animals were in each group. P < 0.05 was taken as statistically significant. FMO: fluorescence minus one.

Figure 4: — Flow cytometry analysis of the expression of NK1R on mouse monocytes and B cells. Monocytes were represented as CD11b⁺Ly-6C/G⁺ cells. B cells were represented as CD19⁺ cells. Mice were randomly selected into OVA-sensitized and OVA-challenged group (OVA + OVA), OVA-sensitized and normal saline (NS) challenged group (OVA + NS), and NS control group (NS + NS). (A) and (B) showed the gating strategies of monocytes and B cells in the blood of OVA-sensitized and non-sensitized mice, respectively. In both (A) and (B), (a) represented gating strategies of monocytes and B cells, while (b) and (c) represented the allergic rhinitis (AR) and allergic asthma (AA) models, respectively. (C) and (D) demonstrated changes in the percentages of NK1R⁺ monocytes of normal saline (NS) and OVA-sensitized AR and AA mice. (E) and (F) illustrated changes in the percentages of NK1R⁺ B cells of NS and OVA-sensitized AR and AA mice. A total of 7 animals were in each group. P < 0.05 was taken as statistically significant. FMO: fluorescence minus one.