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. 2022 Oct 21;22:258. doi: 10.1186/s12866-022-02670-3

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — M. bovis genotype 1 and 2 differences. A Whole genome tree of M. bovis and M. bovoculi constructed in EDGAR using 1220 genes per genome, 1,278,792 bp per genome, with CP087830 as a reference. B Whole genome tree of M. bovis constructed with Parsnp using 74% of CP030241 as a reference and viewed with Ginger. The alleles of 11,070 polymorphisms separate the two genotype clades. ANI values were calculated using the whole genome sequence of M. bovis type strain CCUG 2133 M. Hemolysin protein variant sequences are provided in Supplementary Fig. S1. Isoform sequences of PilA are provided in Supplementary Fig. S3. PilA protein variant sequences connected to serotypes are represented with letters whereas isoforms without corresponding serotypes are represented with numbers