Fig. 3.

A reduction in osi levels affects viability. A Animals carrying a Gal4 insertion interrupting osi produce around 50% of osi levels compared to controls as confirmed by qPCR. For experiments shown in E and F, the number of independent observations is included under each data set; each replica included 30 larvae pooled together. One-way ANOVA was used to analyze statistical significance. B Homozygous osi^Gal4 exhibit a poor performance during larval development, and only around 2% reach adulthood. Animals were counted every 24h until eclosion of controls. A representative experiment (out of two performed) is shown. C qPCR shows that expression of osi^RNAi in an ubiquitous pattern (actG4) decreases osi levels to about 20% of controls. The number of independent observations is included under each data set; each replica included 30 larvae pooled together. A two-tailed T-test was used to analyze statistical significance. D Downregulation of osi (actG4) produces a strong phenotype reminiscent of that of homozygous osi^100B. Animals were measured every 24h until the death of all experimental individuals. osi downregulation stunt growth compared to controls and die between 72 and 96h. Animals were collected from two independent plates, and the number of independent observations is included under each data set. Two-way ANOVA was used to analyze statistical significance. All panels: Shapiro-Wilk test was used for normality assessment (see Additional file 2). Different letters indicate significant differences, p<0.05; treatments sharing any letter are not statistically different. The total number of samples analyzed is mentioned below each column. All datasets and statistical analysis on which the conclusions are based are included in Additional file 2