Representative rep-PCR gels for the 15 ECOR strains, showing fingerprints as generated using the BOXA1R primer (left) or the ERIC2 primer (right), with standard cycling (top) or 65-TD cycling (bottom). Lanes are labeled with strain numbers and bracketed according to ECOR group (A, B1, B2, D, and nonaligned [non]). Lanes M, 250-bp molecular size marker. Note that fingerprints for a given strain as generated with the same primer differ considerably between standard cycling and 65-TD cycling.