TABLE 3.
Net discrimination power of rep-PCR fingerprints from 15 ECOR strains in relation to cycling regimen and use of single versus multiple cyclers
Fingerprints from cyclers A and B analyzed separately or combined | Cycling regimen | Net discrimination power (%)a
|
||
---|---|---|---|---|
ERIC2 fingerprints | BOXA1R fingerprints | Composite fingerprints | ||
Separate | Standardbd | 7.4 | 5.7 | 13.0 |
65-TDbe | 26.7 | 11.9 | 19.7 | |
Combined | Standardcd | −5.2 | −6.1 | −1.1 |
65-TDce | 25.1 | −0.2 | 19.6 |
Larger values for net discriminating power (similarity index − differentiation index) indicate better differentiation. Six replicate fingerprints per strain were included in each analysis.
With A separate from B, for standard versus 65-TD cycling, P < 0.001 (ERIC2), P > 0.10 (BOXA1R), and P = 0.03 (composite).
With A and B combined, for standard versus 65-TD cycling, P < 0.001 (ERIC2 and composite) and P = 0.08 (BOXA1R).
With standard cycling, for A separate from B versus A and B combined, P = 0.04 (ERIC2) and P < 0.001 (BOXA1R and composite).
With 65-TD cycling, for A separate from B versus A and B combined, P > 0.10 (ERIC2 and composite) and P = 0.002 (BOXA1R).