Important amino acid sequence in BRAF protein.
| BRAF protein region | Amino acid sequence | Different binding pocket between N- and C-lobe | Amino acid sequence | |||
|---|---|---|---|---|---|---|
| CR1 | 150–290 | RBD | 155–227 | Glycine-rich ATP-phosphate-binding loop (P-loop) and ribose pocket | 463–471 | |
| CRD | 234–280 | |||||
| CR2 | 360–375 | αC-Helix | 492–504 | |||
| CR3 (protein kinase domain) | 457–717 | RAF selective pocketa | DIF | 504–511 | ||
| DFG motif | 594–596 | |||||
| Gatekeeper residue | T529 | |||||
| Hinge region (adenine region) | 530–535 | |||||
| Catalytic loop | 574–581 | |||||
| Activation segment | 599–623 | |||||
a
Raf selective pocket contain (DIF and DFG motif).