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Effect of time of stimulation on the number of IFN-γ-producing cells. PBMC of HIV-1-infected subject E12 (CD4+ T-cell count, 1,017 cells/mm3; HIV-1 RNA load, <50 copies/ml; treated with 3TC, d4T [stavudine], and IDV) and HIV-1-negative donor L27255 were stimulated with medium alone (negative control), PMA-ionomycin (positive control), VV-Vac, or VV-GPE.
