Table 1.

Summary of Sample Size and Statistical Methods Used for Main Analyses (Total Sample Size: n = 171 Animals)

	Albino	Pigmented	Statistics
Group 1 (G1) (Fig. 1)	hyperopic: n = 17 myopic: n = 17	hyperopic: n = 17	One-way ANOVA with Bonferroni correction
Group 2 (G2) (Figs. 2, 3) (Tables 2, 3)	n = 27	n = 23	One-way ANOVA with Bonferroni correction; Correlation analysis
Proteomics analysis (Fig. 7, Table 4)	n = 4 from G2	n = 4 from G2	Linear models for microarrays algorithm (limma)
Group 3 (G3) FDM from 2 to 4 weeks of age (Fig. 4)	Control: n = 17 FDM: n = 22	Control: n = 10 FDM: n = 21	Correlation analysis; Multivariate linear regressions
Repeated ChT measurement (Fig. 5)	n = 7 from G3	n = 7 from G3	One-way ANOVA with Bonferroni correction

Group 1 guinea pigs were used for cross-sectional, case-control comparisons at P6. After guinea pigs were recruited, they underwent biometric measurements and optical coherence tomography imaging. Group 2 guinea pigs were observed longitudinally from P6 to P14 to assess the correlation between ChT and refraction. Group 3 guinea pigs either underwent FDM induction or served as age-matched controls that underwent natural eye growth. Only the right eyes from guinea pigs undergoing natural growth were included in analyses. FDM was induced in either left or right eye (assigned at random). Of the n = 27 albinos in Group 2, based on P6 baseline refraction, four were myopic, and 23 were hyperopic; whereas at P14 refraction, more GPs naturally progressed to myopia, with 12 myopic, 2 emmetropic and 13 still hyperopic. Of the n = 22 albinos that underwent FDM in Group 3, at P14 baseline refraction, there were seven myopic, one emmetropic, and 14 hyperopic animals.