Skip to main content
. 2022 Oct 14;119(42):e2123338119. doi: 10.1073/pnas.2123338119

Fig. 1.

Fig. 1.

NSUN2 depletion suppresses viral replication and gene expression. NSUN2 depletion suppresses RSV (A–D) and VSV (E–H) replication. A549 cells were transfected with siNSUN2 or siRNA negative control (siNC). After 24 h, cells were infected with GFP-expressing viruses (rgRSV or rVSV-GFP) at an MOI of 0.1, GFP expression was captured by fluorescence microscopy (A and E), and GFP-positive cells were quantified by flow cytometry (B and F). A single-step growth curve shows the release of RSV (C) and VSV (G). Total cell extracts were harvested from rgRSV (D)- or rVSV-GFP (H)–infected A549 cells and subjected to Western blot (D and H). (I) Western blot showing KO of NSUN2 in A549 cells. (J–Q) NSUN2 KO suppresses RSV and VSV replication. NSUN2-KO A549 cells or control sgRNA A549 cells were infected with rgRSV (J–M) or rVSV-GFP (N–Q) at an MOI of 0.1. GFP images were captured (J and N), percent of GFP-positive cells was quantified (K and O), RSV (M) or VSV (Q) protein expression was determined by Western blot, and RSV (L) and VSV (P) titers were determined. All results are from three independent experiments. Data were analyzed using Student’s t test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).