Fig. 5.

m⁵C deficiency-mediated antiviral response is dependent on RIG-I but not MDA5. Confluent WT A549 cells and RIG-I-KO (RIG-I^−/−) A549 cells were transfected with siRNA against NSUN2 or control siRNA, followed by infection with rgRSV at an MOI of 0.1. GFP images were taken by fluorescence microscopy (A) and viral titer in cell culture supernatants was determined by a 50% tissue culture infective dose (TCID₅₀) (B). (C) A549 cells were transfected with siRNA against NSUN2, MDA5, or both, followed by infection with rgRSV at an MOI of 0.1, and viral titer in cell culture supernatants was determined by TCID₅₀ . Data were analyzed using a Student’s t test (*P < 0.05, **P < 0.01, ***P < 0.001).