Fig. 2.

Constitutive CaMKII activity remains unaltered despite the complete turnover of CaMKII. (A) Timeline of the experiment. (B) Two scenarios are shown for the fate of CaMKII activity. (B₁) CaMKII at the beginning of the experiment is illustrated as black circles with phosphates attached. CaMKII synthesized during the 2-wk period is illustrated with open circles. In this scenario the active CaMKII is degraded and activity is lost. (B₂) In this scenario, the old CaMKII phosphorylates newly synthesized CaMKII. (C) After slice culture creation at P6–P8, slices were switched to slice culture media containing APV (100 μM) and nifedipine (20 μM). Recordings were carried out at various time points after the slice media was switched. All recording consisted of a baseline recording followed by changing the ACSF to one containing myr-CN27 (1 μM). Cells were recorded at various time points after solution change. (C) Recordings from days 2–3 after slice media was switched to APV and nifedipine (n = 7). (D) Recordings made at day 7 (n = 5). (E) Recording done on day 14 (n = 7). In all three cases, the myr-CN27 exhibited the same degree of depression.