Fig. 5.

Lead SAH(229–259)_C constructs are cell permeable and nontoxic to T cells. (A) Cellular uptake by human iTreg cells incubated with FITC-labeled SAHs. Hoechst was used as a nuclear dye. SAH uptake exhibits distinct patterns, ranging from diffuse to more punctate fluorescence within the cell, including the nuclei and cytoplasm. (B and C) Absence of disruptive membrane lysis by (B) lack of nonspecific murine thymocyte membrane lysis as measured by LDH release at 4 h and normalized to vehicle and (C) apoptosis as measured by annexin V (APC) and Propidium Iodide (PI) staining and flow cytometry.