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Kinetic parameters for [Na+] dependence of rotation rate of wild type and aE634A
| Protein | Km1Na (mM) | Vmax1Na (rps) | kon1Na* (M−1 s−1) | Km2Na (mM) | Vmax2Na (rps) | kon2Na* (M−1 s−1) |
|---|---|---|---|---|---|---|
| EhVoV1 wild type | 0.29 ± 0.09 | 15.7 ± 1.6 | 5.4 × 105 | 160.2 ± 88.8 | 35.0 ± 7.1 | 2.2 × 103 |
| EhVoV1 aE634A | 0.32 ± 0.28 | 1.1 ± 0.3 | 3.4 × 104 | 58.3 ± 26.5 | 4.4 ± 0.5 | 7.5 × 102 |
[ATP] was 5 mM.
*The binding rate constant of Na+ estimated by kon1(2)Na = 10 × Vmax1(2)Na/Km1(2)Na.
