Skip to main content
. Author manuscript; available in PMC: 2023 Oct 1.
Published in final edited form as: Shock. 2022 Aug 18;58(4):287–294. doi: 10.1097/SHK.0000000000001979

Figure 2. FMT treatment prevents the expansion of the proportion of T cells after traumatic brain injury in mice.

Figure 2.

Immune cells were isolated from mouse groups using Fluorescent Activated Cell Sorting and assessed via single-cell RNA sequencing for cell type. Immune cell proportions are shown here for all groups: Sham (untreated, no injury), TBI (Untreated, with injury), FMT (Treated, No injury ((sham)), TBI-FMT (Treated, with injury). A) UMAP plot demonstrating clustering obtained for each group (Sham, TBI, FMT, FMT-TBI) two biological replicates were combined. Cluster annotations: MG (microglia), T (T-cells) B (B-cells) NK (natural killer cells), NP (neutrophils), Mo_MΦ (monocytes/monocytes-derived macrophages), EC (epithelial cells). Number of total cells obtained from each group: Sham=3952, TBI=5570, FMT-Sham=3286, FMT-TBI=5832. B) Pie charts demonstrating the proportion of the identified cell types across samples. FMT mitigated the expansion in the proportion of T cells within the brain after TBI. C) Dim plots showing markers that identify each cell type. Average expression and percent expression are indicated by dot size.