Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-vGluT1 | Millipore | Cat. No. AB5905 | 1:1000 |
| Antibody | Anti-Homer1 | Millipore | Cat. No. ABN37 | 1:1000 |
| Antibody | Anti-GAD65 | DSHB | Cat. No. mGAD6-a | 1:500 |
| Antibody | Anti-MAP2 | Millipore | Cat. No. AB5622 | 1:1000 |
| Antibody | Anti-Synaptotagmin 1 | Südhof lab | CL41.1 | 1:1000 |
| Antibody | Anti-Neurexin | Südhof lab | G394 | 1:500 |
| Antibody | Anti-CASK | BD Transduction Laboratories |
Cat. No. 610782 | 1:1000 |
| Antibody | Anti-PSD95 | Südhof lab | L667 | 1:500 |
| Antibody | Anti-Synapsin | Südhof lab | E028 | 1:1000 |
| Antibody | Anti-Neuroligin-1 | Südhof lab | 4F9 | 1:500 |
| Antibody | Anti-β-actin | Sigma | Cat. No. A1978 | 1:10000 |
| Antibody | Anti-Calbindin | Sigma | Cat. No. C9848 | 1:2000 |
| Sequence- based reagent |
Cbln1 in-situ probe | Advanced Cell Diagnostics |
Cat. No. 538491-C2 | |
| Sequence- based reagent |
Cbln2 in-situ probe | Advanced Cell Diagnostics |
Cat. No. 428551 | |
| Recombinant DNA reagent | Lenti-hSyn-Cre-eGFP | Aoto et al., 2013 | N/A | Lentiviral construct to express Cre and eGFP |
| Recombinant DNA reagent | Lenti-hSyn-eGFP | Aoto et al., 2013 | N/A | Lentiviral construct to express eGFP |
| Recombinant DNA reagent | Lenti-CAG-Cre-eGFP | This paper | N/A | Lentiviral construct to express Cre and eGFP |
| Recombinant DNA reagent | Lenti-CAG-eGFP | This paper | N/A | Lentiviral construct to express eGFP |
| Recombinant DNA reagent | pAAV-hSyn-Cre-eGFP | Aoto et al., 2015 | N/A | AAV construct to express Cre and eGFP |
| Recombinant DNA reagent | pAAV-hSyn-eGFP | Aoto et al., 2015 | N/A | AAV construct to express eGFP |
| Recombinant DNA reagent | pAAV-hSyn-eGFP-p2A-Nrxn1βSS4+/- | Dai et al., 2019 | N/A | AAV construct to express eGFP and Nrxn1βSS4+/- |
| Recombinant DNA reagent | pAAV-hSyn-eGFP-p2A-Nrxn3βSS4+/- | Dai et al., 2019 | N/A | AAV construct to express eGFP and Nrxn3βSS4+/- |
| Chemical compound, drug | CNQX | Tocris | Cat. No. 0190 | |
| Chemical compound, drug | Picrotoxin | Tocris | Cat. No. 1128 | |
| Chemical compound, drug | TTX | Fisher Scientific | Cat. No. 50-753-2807 | |
| Chemical compound, drug | Biocytin | Sigma | Cat. No. B4261 | |
| Chemical compound, drug | Streptavidin Alexa 647 | Thermo Fisher | Cat. No. S32354 | 1:1000 |
| Genetic reagent (Mus musculus) | Mouse: C57BL/6J wildtype | The Jackson Laboratory | Jax Stock no: 000664 | |
| Genetic reagent (Mus musculus) | Mouse: Nrxn1-SS4+, Nrxn3-SS4+cKI |
Dai et al., 2019; Aoto et al., 2013 | N/A | |
| Genetic reagent (Mus musculus) | Mouse: Cbln1, Cbln2, Cbln12 cKO, Cbln2 KO |
Seigneur and Südhof, 2017 | N/A | |
| Software, algorithm | Clampfit 10 | Molecular Devices | https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite | |
| Software, algorithm | Igor software | Wavemetrics | https://www.wavemetrics.com/downloads | |
| Software, algorithm | Image Studio | LI-COR Biosciences | https://www.licor.com/bio/image-studio/ | |
| Software, algorithm | NIS-Elements AR Analysis |
Nikon | https://www.microscope.healthcare.nikon.com/products/software/nis-elements/nis-elements-advanced-research | |
| Software, algorithm | Viewer III | Bioserve | http://www.biobserve.com/behavioralresearch/products/viewer/ | |
| Software, algorithm | Prism | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ | |
| Software, algorithm | SigmaPlot | Systat Software | https://systatsoftware.com/sp/download.html |