Figure 1.

Ogfod1 ablation alters the cardiac proteome. (A) Schematic of workflow in isolating hearts from Ogfod1-WT and Ogfod1-KO hearts for proteomics. (B and C) Ingenuity^® Pathway Analysis (IPA^®) analysis on the 138 significantly increased proteins (B), and the 65 significantly decreased proteins (C) identified by mass spectrometry. Bar length represents the significance with which each pathway was identified on the –log(P-value) scale. n = 5 biological replicates per group. Student’s t-test was used to determine significance between groups. A P-value of <0.05 was considered significant. HPLC-MS/MS, high-performance liquid chromatography tandem mass spectrometry.