Figure 4.

Ogfod1 ablation leads to protection against ischaemia–reperfusion injury ex vivo. (A) Langendorff-perfused hearts from WT and KO mice were subjected to ischaemia and reperfusion protocol as shown in the schematic. (B) Rate pressure product (RPP) recovery following ischaemia and reperfusion. (C) Infarct size measured at the end of the ischaemia–reperfusion period. Data shown as mean ± standard error. n = 8 biological replicates per group. Student’s t-test with Welch’s correction was used to determine significance between groups. A P-value of <0.05 was considered significant. LVDP, left ventricular developed pressure; TTC, 2,3,5-triphenyltetrazolium chloride.