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. 2022 Oct 19;13(1):1868–1883. doi: 10.1080/21505594.2022.2135273

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Identification and confirmation of OmpR-regulated genes.

A) RNA-seq results representation of differentially expressed genes between AB5075 wildtype and its ΔompR mutants. Sixty-five OmpR-activated candidate genes (<-2 log₂ fold change, blue zone) and 71 OmpR-repressed candidate genes (>2 log₂ fold change, green zone) were identified. B) The RNA-seq results were combined with a Tn-seq dataset conducted to identify A. baumannii genes that are important or deleterious for A. baumannii virulence [26]. Nineteen OmpR-activated candidate genes were important for A. baumannii virulence whereas 12 OmpR-repressed candidate genes were deleterious for A. baumannii virulence. RR: read ratio. C) The transcript levels were determined in A. baumannii AB5075 wildtype, AB5075 ΔompR (white), AB5075 OmpR D71E (light grey), AB5075 OmpR D71A (dark grey) and AB5075 OmpR R198L (black) mutants by quantitative real-time PCR and the expression level was normalized to the expression of the AB5075 wildtype strain (means ± SEM of two technical replicates). D) The transcript levels of ABUW_RS02965 (white) and ABUW_RS13110 (black) were determined in A. baumannii ΔompR mutants and normalized to the transcript levels of their respective wildtype strains (means ± SEM of two technical replicates). Horizontal dotted lines depict a 2-fold up- or downregulation.