Table 5.
Sensitivity analyses for the top hit of the main meta-analysis (cg23757341).
| N | Effecta | SEa | P value | Direction | I2 | |
|---|---|---|---|---|---|---|
| Main meta-analysis | ||||||
| rMEDp fully adjusted | 2802 | 0.0638 | 0.0114 | 2.41E-08 | ++++++ | 50.6 |
| rMED fully adjusted | 2802 | 0.0601 | 0.0114 | 1.19E-07 | ++++++ | 48.8 |
| Main meta-analysis | ||||||
| rMEDp minimally adjusted | 2802 | 0.0500 | 0.0109 | 4.73E-06 | ++++++ | 52.1 |
| rMED minimally adjusted | 2802 | 0.0467 | 0.0108 | 1.45E-05 | ++++++ | 51.5 |
| Sensitivity analyses for rMEDp fully adjusted | ||||||
| European ancestry only (ALSPAC, Generation R, Healthy Start NHW, INMA, Project Viva) | 2671 | 0.0660 | 0.0119 | 2.67E-08 | +++++ | 58.4 |
| Cohorts using FFQ only (ALSPAC, Generation R, INMA, Project Viva) | 2414 | 0.0491 | 0.0133 | 2.21E-04 | ++++ | 38.8 |
| European cohorts only (ALSPAC, Generation R, INMA) | 2088 | 0.0378 | 0.0156 | 1.51E-02 | +++ | 0 |
| American cohorts only (Healthy Start Hispanic, Healthy Start NHW, Project Viva) | 714 | 0.0939 | 0.0168 | 2.31E-08 | +++ | 16.5 |
| Leave-one-out analyses for rMEDp fully adjusted | ||||||
| Excluding ALSPAC | 2142 | 0.0680 | 0.0120 | 7.90E-09 | +++++ | 49.7 |
| Excluding Generation R | 1754 | 0.0682 | 0.0139 | 8.61E-07 | +++++ | 59.2 |
| Excluding Healthy Start Hispanic | 2671 | 0.0660 | 0.0119 | 2.67E-08 | +++++ | 58.4 |
| Excluding Healthy Start Non Hispanic White | 2545 | 0.0522 | 0.0128 | 4.43E-05 | +++++ | 33.4 |
| Excluding INMA | 2422 | 0.0714 | 0.0124 | 7.56E-09 | +++++ | 46.5 |
| Excluding Project Viva | 2476 | 0.0550 | 0.0127 | 1.25E-05 | +++++ | 48.8 |
aEffect size and SE are presented as percentage change in DNA methylation per 1-point increase in the rMEDp score.
NHW, non-Hispanic white.
EWAS was adjusted for sex + maternal educational level + maternal age + maternal smoking + maternal BMI + maternal total energy intake + batch + cell types.