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. 2022 Oct 18;13(1):1849–1867. doi: 10.1080/21505594.2022.2136433

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — The YLMY motif regulates the protein expression of AP complexes. BSR-T7/5 cells were infected with an NDV containing a wildtype (rSG10*) or mutant (rSG10*-F/Y524A, rSG10*-F/Y527A, or rSG10*-F/Y524AY527A) YLMY motif, at a multiplicity of infection of 0.1. (a) the expression levels of each F protein and host AP1M1 and AP2M1 at various post-infection time points were determined by western blotting. (b) Protein expression levels are expressed as percentages of the levels for rSG10*, which were set at 100%. The p-values were calculated by a two-way ANOVA; *p < 0.05; **p <0.01; ***p <0.001; n = 3.