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. 2022 Oct 18;13(1):1849–1867. doi: 10.1080/21505594.2022.2136433

Figure 5.

Figure 5.

The viral F protein is transported by AP-1 and AP-2. (a) Expression of AP1M1 and AP2M1 in the membrane protein fraction was determined by western blot. BSR-T7/5 cells were infected with an NDV containing a wildtype (rSG10*) or mutant (rSG10*-F/Y524A, rSG10*-F/Y527A, or rSG10*-F/Y524AY527A) YLMY motif, at a multiplicity of infection of 0.1. The membrane protein was extracted, following the kit instructions, at different post-infection time points. (b) Quantitative analysis of AP1M1, AP2M1, and viral F protein expression levels, expressed as percentages of the levels for rSG10* (set at 100%). (c) a co-IP conducted on the membrane proteins. After cells were infected with an NDV containing a wildtype or mutant YLMY motif, the membrane protein fraction was extracted and used in an immunoprecipitation performed with an anti-F antibody. (d) TEM micrographs of infected cells at 36 hpi. White arrows mark the presence of many lysosomes and autophagosomes, and black arrows indicate transport vesicles in the cytoplasm. N, nucleus; ER, endoplasmic reticulum.