Figure 6.

Treatment with siAP1M1 or siAP2M1 impairs the expression and transportation of mutant F protein. (a) at 24 h after cells were transfected with siAP1M1 or siAP2M1, they were infected with an NDV containing a wildtype (rSG10*) or mutant (rSG10*-F/Y524A, rSG10*-F/Y527A, or rSG10*-F/Y524AY527A) YLMY motif. The F protein expression level in these cells was measured by western blot. (b) the proteins of cells treated as above were separated into membrane and cytoplasm fractions before being subjected to a western blot. (c) Quantitative analysis of the viral F protein expression from part (b). Protein expression levels are presented as percentages of the β-actin expression level. The p-values were calculated by a two-way ANOVA; *p <0.05; **p <0.01; ***p <0.001; n = 3.