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. 2022 Oct 18;13(1):1849–1867. doi: 10.1080/21505594.2022.2136433

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — The expression of p-AP2M1, AAK1, and GAK in cells infected with an NDV containing a wildtype or mutated YLMY motif. Cells were infected with an NDV containing a wildtype (rSG10*) or mutated (rSG10*-F/Y524A, rSG10*-F/Y527A, or rSG10*-F/Y524AY527A) YLMY motif. (a) the phosphorylation of AP2M1 in these cells was detected by western blot. (b) the mRNA expression levels of viral NP and F genes and host AAK1 and GAK genes in these cells were analysed by RT-qPCR. The p-values were calculated by a two-way ANOVA; *p <0.05; **p <0.01; ***p <0.001; n = 3. (c) Protein expression of host AAK1 and GAK in these cells.