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. 2022 May 3;17(11):1513–1534. doi: 10.1080/15592294.2022.2069386

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 1. — Macroscopic and histopathological analyses of rat livers upon methyl donor deficient diet. (a) Average body weights of healthy rats on choline-sufficient diet (CSAA) and rats on carcinogenic choline-deficient diet (CDAA) over 52-week experimental timeline. Bar graph represents average final body weights of CSAA and CDAA groups at the 52-week time-point. (b) Average liver weights and relative liver weights of CSAA and CDAA groups at the 52-week time-point. (c) Representative histopathological images at 4x and 20x magnification of whole livers from CSAA and CDAA groups. (d) Number of liver nodules in CSAA and CDAA livers with representative images of livers from rats in CSAA and CDAA groups. Results represent mean ± SD, n = 6 rats per group, **P < 0.01.