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. 2022 Oct 14;2022:4769385. doi: 10.1155/2022/4769385

Figure 4.

Figure 4

miR-29b targeted ROBO1 and SRGAP2. (a) Base pairings of the ROBO1 3′UTR and SRGAP2 3′UTR with miRNA-29b as verified by the StarBase website (http://starbase.sysu.edu.cn/). (b) Quantification of luciferase activity in HUVECs transfected with the ROBO1 3′UTR and SRGAP2 3′UTR to form pGL3-luciferase reporter constructs with the miRNA-29b mimic or mimic NC. (c) RT-qPCR analysis of ROBO1 and SRGAP2 expression in HUVECs incubated with miRNA-29b overexpressing BxPC3 and AsPC-1 cells that had been treated or not treated with GW4869. (d) Western blot study to determine the expression of ROBO1 and SRGAP2 proteins in HUVECs incubated with miRNA-29b overexpressing BxPC3 and AsPC-1 cells that had been treated or not treated with GW4869. ∗∗∗P < 0.001 vs. NC; ###P < 0.001 vs. mimics+GW489. Experiments were repeated for three times and presented as mean ± SD.