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. 2022 Oct 21;11(10):e1421. doi: 10.1002/cti2.1421

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© 2022 The Authors. Clinical & Translational Immunology published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology, Inc.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Binding of ACE2‐Bite to SARS‐CoV‐2 Spike protein variants on pseudotyped lentiviruses for virus neutralisation. (a) Virus neutralisation assay. ACE2‐Bite and Spike (SARS‐CoV, SARS‐CoV‐2, Delta and Omicron) pseudotyped lentiviruses are pre‐incubated and then added to ACE2‐overexpressing 293 cells. (b) Virus neutralisation data of the lentiviruses pseudotyped with different Spike proteins that pre‐incubated with ACE2‐Bite at different ACE2‐Bite:virus ratios then added to ACE2‐overexpressing 293s. The experiments were replicated twice with similar results.