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. 2022 Sep 24;41(43):4823–4838. doi: 10.1038/s41388-022-02451-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A, B Survival analyses of overall survival (OS) and relapse-free survival (RFS) were performed based on Kaplan–Meier curves in oncomir database. C Expression of miR-3648 was determined by qPCR in 82 human gastric tissues, which was normalised against an endogenous U6 RNA control. NT normal mucosal tissues, GC GC tissue. ***P < 0.01, paired t-test. D Relative miR-3648 levels of GC and normal tissues measured by qPCR were shown using a waterfall plot. Error bars represent the mean ± SD from three independent experiments. E miR-3648 expressions in eight gastric epithelial cell lines by qPCR. One-way ANOVA and Dunnett’s T3 multiple comparison test. ****P < 0.001 between normal and cancer gastric epithelial cells. The result is presented as the mean ± SD of three independent experiments. F, G The relationship between miR-3648 expression and clinical stages or local invasion. ***P < 0.01; ****P < 0.001. P values were estimated using two-tailed unpaired Student’s t-test. H ISH analysis of miR-3648 expression level in normal gastric mucosa and GC tissues. Scale bars, 100 μm in H.