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. 2022 Sep 24;41(43):4823–4838. doi: 10.1038/s41388-022-02451-2

Fig. 4. FRAT1 or FRAT2 mediate the effects of miR-3648 on tumour invasion and metastasis in GC cells.

Fig. 4

A The migration ability and B the invasion ability of cells transfected with miR-3648 mimics and/or FRAT1 or FRAT2 expression plasmid were determined using a wound-healing or the transwell assay. **P < 0.05; ***P < 0.01; ****P < 0.001. The result is presented as the mean ± SD. P values were estimated using two-tailed unpaired Student’s t-test. All experiments were repeated at least three times with identical findings. C The GC MKN45 cells were orthotopically transplanted into the lung of nude mice and mice were randomly divided into seven groups as follows: m-NC, miR-3648, Vector, FRAT1, FTAT2, miR-3648 + FRAT1 and miR-3648 + FRAT1 group (n = 3 in each group, one of three nude mice was showed). Error bars represent the mean ± SD. P values were estimated using two-tailed unpaired Student’s t-test. D The mice were sacrificed and metastatic cancer tissues were stained with haematoxylin and eosin (H&E). E Representative images of metastatic loci in the lungs were shown. Number of metastatic loci in the lungs was counted. **P < 0.05; ***P < 0.01; ****P < 0.001. F Immunohistochemical (IHC) staining of MMP2 and MMP9 expression. Scale bars, 50 μm in B, 200 μm in D and 100 μm in F.